# ROLES OF POLYPEPTIDE GROWTH FACTORS IN CELL GROWTH

> **NIH NIH R01** · SAINT LOUIS UNIVERSITY · 1992 · $225,617

## Abstract

The broad goal of this research is to define the roles of
polypeptide growth factors, including platelet-derived growth
factor (PDGF) and brain-derived growth factor (BDGF) in the
regulation of growth of normal and transformed cells.  This
proposal has four aims:

1)  To define the autocrine role of PDGF-like molecules (p28 v-sis
and c-sis gene product) in the growth of cells transformed by
infection with simian sarcoma virus (SSV) and by transfection with
a cDNA clone of the c-sis gene.  Differences in expression of
these PDGF-like molecules will be examined in cells with varying
degrees of tumorigenicity.  Intracellular PDGF-like molecules will
be measured with enzyme-linked immunosorbent assay.
Intracellular biosynthesis and processing will be studied by
Northern gel analysis of transcripts, metabolic labeling,
subcellular fractionation, and electron microscopy.
2)  To define the differences in biosynthesis, processing, and
turnover of the PDGF receptor in SSV- and c-sis-transformed
cells and in normal cells.  The bio-synthesis, processing, and
turnover of the PDGF receptor will be analyzed by
immunoprecipitation of metabolically labeled cells.  PDGF-
receptor negative cells derived from transformed cells will be
selected to test the obligatory role of the receptor in
transformation of these transformed cells.
3)  To study the cell biology of BDGF in neuroblastoma cells.  The
biosynthesis and secretion will be studied by immunoprecipitation
of metabolically labeled cells using anti-BDGF antibodies.  The
effects of anti-BDGF antibodies on the growth of neuroblastoma
cells in culture and in athymic nude mice will be examined.
4)  To isolated and characterize the BDGF receptor from bovine
uterus and to study the biochemistry (protein tyrosine kinase
activity) and biological properties.  Isolation of a significant
quantity of the receptor will permit preparation of antibodies to
the receptor and studies of biosynthesis and turnover of the
receptor in normal and transformed cells, including neuroblastoma
cells.

These studies should provide insight into the roles of PDGF and
BDGF in the regulation of normal cell growth and may help
understand the alteration of this growth regulation that leads to
some forms of cancer.

## Key facts

- **NIH application ID:** 3177142
- **Project number:** 5R01CA038808-08
- **Recipient organization:** SAINT LOUIS UNIVERSITY
- **SAM.gov UEI:** JNBLLTBTLLD8
- **Principal Investigator:** JUNG S. HUANG
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $225,617
- **Award type:** 5
- **Project period:** 1984-12-01 → 1992-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177142

## Citation

> US National Institutes of Health, RePORTER application 3177142, ROLES OF POLYPEPTIDE GROWTH FACTORS IN CELL GROWTH (5R01CA038808-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177142. Licensed CC0.

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