# PROPERIES OF A LYMPHOMA CELL TYROSINE PROTEIN KINASE

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1990 · $132,505

## Abstract

PROPERTIES OF A LYMPHOMA CELL TYROSINE PROTEIN
KINASE: There is increasing evidence that tyrosine protein
kinases have an important role in T lymphocyte activation and
cell cycle progression.  We have identified a tyrosine protein
kinase of apparent molecular weight of 56,000 (pp56Tcell).  This
enzyme is specific to T lymphoctyes and undergoes
phosphorylation by protein kinase C during T lymphocyte
activation.  The general goal of this application is to determine
the function of pp56Tcell in T cell activation.  We have developed
highly sensitive immunoblotting techniques that allow us to
measure the activation pp56Tcell and other tyrosine protein
kinases as well as the activation of protein kinase C.  These
techniques allow us to study these kinases in T cells and
determine if their activation correlates with physiological
responses.  Thus the role of these enzymes will be evaluated in
the response of T cells to a wide variety of mitogenic agents.  The
regulation of pp56Tcell is complex and involves phosphorylation at
several sites.  These phosphorylation reactions will be studied in
detail in order to understand their function and the mechanisms
that underlie their regulation.  The biosynthetic pathway of
pp56Tcell will be characterized to determine if this is regulated
by agents that alter the phosphorylation of pp56Tcell.  The studies
on pp56Tcell will be aided by the production of monoclonal
antibodies to pp56Tcell.  Antibodies to phosphotyrosine have
permitted us to identify a large number of potential substrates for
pp56Tcell.  These substrates will be purified and characterized in
order to determine the function of the tyrosine phosphorylation
that we have observed in T cells.  The results of this research may
provide new insights into the biochemical events involved in the
regulation of the activation and growth of normal and malignant
cells of the T lymphocyte lineage.

## Key facts

- **NIH application ID:** 3177176
- **Project number:** 5R01CA038821-06
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** JOHN E CASNELLIE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $132,505
- **Award type:** 5
- **Project period:** 1985-01-01 → 1993-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177176

## Citation

> US National Institutes of Health, RePORTER application 3177176, PROPERIES OF A LYMPHOMA CELL TYROSINE PROTEIN KINASE (5R01CA038821-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177176. Licensed CC0.

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