# ONCOGENE REGULATION IN MALIGNANT CELLS

> **NIH NIH R01** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 1987 · $178,654

## Abstract

Preliminary findings indicate that human cervical carcinoma cells of the
D98AH2 (D98) cell line, derived from HeLa cells, contain high levels of
mRNA of several oncogenes.  Hybrids of D98 cells fused with normal human
diploid cells initially contain all or most of the chromosomes of both
parental cells and make much less mRNA of some of these oncogenes than the
D98 cells do.  When these hybrids lose chromosomes they again contain
elevated levels of mRNA of these oncogenes.  One chromosome No. 11 of the
diploid parent has almost always been lost from these hybrids, as indicated
by DNA restriction fragment length polymorphic chromosome marker studies,
and they have often also lost copies of the No. 2, the cell parental origin
of which has not yet been determined.  These results suggest that
information of specific chromosomes of the normal cell may affect
transcription of oncogenes.

The objective of this study is to determine if genetic information on
specific chromosomes affects oncogene transcription.  We will conduct
segregation analysis of appropriate panels of cell hybrids and identify
chromosomes that affect the level of transcription of oncogenes to
determine if the postulated regulators are on the same chromosomes as the
structural loci of the oncogenes whose expression they control.

To determine if the findings in hybrids made with the long established D98
cells are represenative of oncogene regulation in other cells, they will be
compared with results from hybrids that will be made with a recently
isolated human cancer cell line.  These cells have a single chromosome
rearrangement with an otherwise normal chromosome complement.  (X)

## Key facts

- **NIH application ID:** 3177334
- **Project number:** 5R01CA038901-03
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** HAROLD P KLINGER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $178,654
- **Award type:** 5
- **Project period:** 1985-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177334

## Citation

> US National Institutes of Health, RePORTER application 3177334, ONCOGENE REGULATION IN MALIGNANT CELLS (5R01CA038901-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177334. Licensed CC0.

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