# PDGF MODULATED NUCLEAR PROTEIN

> **NIH NIH R01** · CHILDREN'S HOSP OF PHILADELPHIA · 1987 · $130,729

## Abstract

The platelet derived growth factor (PDGF) stimulates BALB/c-3T3 cells to
rapidly (within 40 min) and selectively synthesize pI, a 29K dalton nuclear
protein.  The preferential synthesis of pI is noted long before the onset
of DNA synthesis (12 hrs) and may be required for optimal entry of PDGF
treated cells into the S phase.  Spontaneously transformed BALB/C-3T3
cells, which do not require PDGF for growth, synthesize pI in a
constitutive fashion.  pI mRNA has been assayed using cell-free
translation.  It accumulates within 30 min of PDGF treatment and is not
inhibited, but rather, is superinduced by addition of cycloheximide.  Thus,
the properties of pI are remarkably similar to two PDGF modulated
proto-oncogene products, c-myc and c-fos.  The c-myc and c-fos mRNAs are
induced by PDGF within one hr and are superinduced by addition of
cycloheximide.  Furthermore, pmyc and pfos are nuclear proteins.  At
present, pI cannot be adequately studied because appropriate
tools--specific antisera and recombinant DNA clones--do not exist.  This
project will provide these tools to allow a future analysis of pI
function.  pI will be purified to homogeneity for the purpose of preparing
monospecific heterosera.  These heterosera will be utilized for quantifying
the pI translation product in a cell-free system.  mRNA will be isolated
from PDGF-treated BALB/c-3T3 cells and fractionated on the basis of size; a
population enriched in pI sequences will be selected and cloned into the
experssion vectors pUC8/pUC9.  Alternatively the labmda gt 11 experssion
vector system may be used.  Recombinant cDNA clones will be selected by
screening for the antigenic pI fusion protein, or by screening for the pI
cDNA insert using a labeled cDNA probe prepared from pI enriched mRNA
immunoprecipitated from polysomes.  The isolation of a clone containing pI
cDNA will allow the characterization and quantitation of pI mRNA.  (K)

## Key facts

- **NIH application ID:** 3177437
- **Project number:** 5R01CA038939-03
- **Recipient organization:** CHILDREN'S HOSP OF PHILADELPHIA
- **SAM.gov UEI:** G7MQPLSUX1L4
- **Principal Investigator:** CHARLES D SCHER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $130,729
- **Award type:** 5
- **Project period:** 1985-09-01 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177437

## Citation

> US National Institutes of Health, RePORTER application 3177437, PDGF MODULATED NUCLEAR PROTEIN (5R01CA038939-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177437. Licensed CC0.

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