# A ACETYLATION PHARMACOGENETICS:ARYLAMINES AND DNA DAMAGE

> **NIH NIH R01** · UNIVERSITY OF MICHIGAN AT ANN ARBOR · 1989 · $139,875

## Abstract

We have proposed a pharmacogenetic investigation of arylamine drugs,
carcinogens, and other environmental chemicals in genetically defined
animal models, and in tissues and cells isolated from them.  In this study,
we will determine the extent and significance of nuclear DNA damage caused
by exposure of cells and tissues to the polymorphically acetylated model
arylamine carcinogen, 2-aminofluorene.  We will develop congenic mouse
lines that possess different combinations of acetylator and Ah-inducible
phenotypes for this purpose.  We will determine DNA damage in isolated
hepatocytes and urothelial cells by examining unscheduled DNA synthesis and
DNA adducts; we will also determine DNA damage in vivo by examining DNA
synthesis and DNA adducts in liver and urinary bladder of mice after short
term administration of 2-aminofluorene.  Furthermore, we will determine the
metabolic profiles of 2-aminofluorene in isolated cells and intact mice.
Our objective is to assess the importance of these hereditary metabolic
polymorphisms to arylamine-induced DNA damage.

## Key facts

- **NIH application ID:** 3177645
- **Project number:** 5R01CA039018-05
- **Recipient organization:** UNIVERSITY OF MICHIGAN AT ANN ARBOR
- **SAM.gov UEI:** GNJ7BBP73WE9
- **Principal Investigator:** WENDELL W WEBER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $139,875
- **Award type:** 5
- **Project period:** 1985-01-01 → 1990-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177645

## Citation

> US National Institutes of Health, RePORTER application 3177645, A ACETYLATION PHARMACOGENETICS:ARYLAMINES AND DNA DAMAGE (5R01CA039018-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177645. Licensed CC0.

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