# PROTON NMR ASSESSMENT OF RADIO AND CHEMOTHERAPY EFFECTS

> **NIH NIH R01** · CLEVELAND CLINIC FOUNDATION · 1988 · $32,556

## Abstract

Proton spin-lattice and spin-spin relaxation times(T1 and T2, respectively)
of cells and tissues are mainly affected by free or bulk water molecules in
the intra-and extra-cellular environment and hydration water molecules
which interact with macromolecules.  It is assumed that, to a first
approximation, the reciprocal of T1 (or T2) of cells or tissues is the
linear sum of the reciprocals of T1 (or T2) of these water components.
Thus, we hypothesize that change in the ratio of the amount of hydration
water to that of bulk water or structural change in conformation of
macromolecules in a biological system may lead to an alternation of its
apparent proton relaxation times.  These biological changes may occur in
cells and tumor due to physiological constraints or to damage caused by
radiation and chemotherapeutic drugs.

In this proposed study, we examine the proton relaxation times of mammalian
cells which are present in a variety of physiological conditions (e.g.
different phases in the division cycle, logarithmic- and plateau-phase
growth. amd quiescent and proliferative states).  We also measure T1 and T2
of mouse solid tumors and their neoplastic and host subpopulations.
Following these background studies, we will investigate the correlations of
T1 and T2 with radiation- and adriamycin-induced cell-age redistribution,
G2 accumulation, and necrotic processes in the same biological systems.  In
addition, the change of T1 and T2 relative to transition of quiescent
population to proliferative one will be examined.  Finally, the radiation
effects in tumors will be further studied by in vivo NMR imaging and
subsequent analysis of the T1 and T2 so derived.  The goal of these
systematic studies is to establish the potential utilization of proton NMR
relaxation techniques in probing radiation damage, and the extent to which
NMR imaging is sensitive to the related applications.

## Key facts

- **NIH application ID:** 3177675
- **Project number:** 3R01CA039032-03S1
- **Recipient organization:** CLEVELAND CLINIC FOUNDATION
- **SAM.gov UEI:** TDGUJ1WA4UJ9
- **Principal Investigator:** FRANK Q. NGO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $32,556
- **Award type:** 3
- **Project period:** 1984-12-01 → 1989-09-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177675

## Citation

> US National Institutes of Health, RePORTER application 3177675, PROTON NMR ASSESSMENT OF RADIO AND CHEMOTHERAPY EFFECTS (3R01CA039032-03S1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177675. Licensed CC0.

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