# CELL SURFACE ANTIGENS OF MURINE TUMORS

> **NIH NIH R01** · COLORADO STATE UNIVERSITY · 1988 · $149,399

## Abstract

Cell surface glycoproteins that are coded for by genes within the MHC are
known to be critically important to a variety of immune and nonimmune
cell-cell interactions.  We recently found that some tumor cells express
histocompatibility antigens different from those on syngeneic normal
cells.  We intend to exploit this system to investigate three aspects of
the biology of H-2 antigens:  (1) the frequency of occurrence and origin of
modified H-2 antigens on tumor cells; (2) the relationship between altered
H-2 antigens and the malignant phenotype; and (3) the bases of mechanisms
which operate at the genetic level to maintain homology among H-2 antigens.

To investigate the frequency of occurrence and origin of tumors expressing
altered H-2 antigens, our research will induce additional tumors.  These
tumor cells will be characterized in both early and late passages and
compared with cells derived from the primary host.  These studies will
allow us to assess the frequency of tumors with modified H-2 antigens, the
effects of these antigens on the growth properties of the tumor, and the
influence of continuous passage and host chimerism on the appearance of
tumors with altered histocompatibility antigens.  In addition, DNA-mediated
gene transfer will be used to investigate the relationship between
malignant transformation and the appearance of altered H-2 antigens.
Finally, we will perform in-depth analyses of the chemical structure of
both the H-2 antigens themselves and the genes which encode these cell
surface glycoproteins.  The results obtained in these studies will allow us
to determine precisely which regions of the H-2 molecule have been affected
and to identify the nature and probable origin of the underlying genetic
changes.  The information generated by these coordinated studies will
provide greater insight into the function of H-2 antigens in host-tumor
interactions and the influence of altered H-2 antigens on the malignant
phenotype.  Furthermore, these studies will provide a better understanding
of the relationship between mechanisms that generate H-2 antigen diversity
and mechanisms which maintain homology within this gene family.  More
generally, these studies should significantly facilitate understanding and
manipulation of eukaryotic genetics and the mammalian immune response.  (AG)

## Key facts

- **NIH application ID:** 3177758
- **Project number:** 5R01CA039054-04
- **Recipient organization:** COLORADO STATE UNIVERSITY
- **SAM.gov UEI:** LT9CXX8L19G1
- **Principal Investigator:** GERALD N CALLAHAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $149,399
- **Award type:** 5
- **Project period:** 1984-08-01 → 1989-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177758

## Citation

> US National Institutes of Health, RePORTER application 3177758, CELL SURFACE ANTIGENS OF MURINE TUMORS (5R01CA039054-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177758. Licensed CC0.

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