# EMBRYONAL CARCINOMA GROWTH AND DIFFERENTIATION

> **NIH NIH R01** · DUKE UNIVERSITY · 1993 · $230,452

## Abstract

This research is an extension of previous work using polyoma mutant viruses
and viral sequences with recombinant DNA probes to study the gene
expression requirements of teratocarcinoma cells.  Teratocarcinoma cells
consist of a stem-cell embryonal carcinoma (EC), which can be grown as a
cell line under cell culture conditions, and then depending upon the cell
line used, can be induced to differentiate to other cell types.  We are
using the viral and recombinant DNA probes to examine what DNA sequences
are necessary for expression of genes in these different cell types and to
try to select for cellular sequences which might play a role in cell
type-specific gene expression.

This work makes use of various gene transfer techniques, manipulation of
small restriction fragments containing viral enhancement sequences,
oligonucleotide synthesis to reconstruct regulatory sequences, and
competition transfection experiments to examine whether regulatory
sequences are competing for cellular factors, which play a role in
enhancing gene expression in teratocarcinoma cells.  (G)

## Key facts

- **NIH application ID:** 3177812
- **Project number:** 5R01CA039066-10
- **Recipient organization:** DUKE UNIVERSITY
- **SAM.gov UEI:** TP7EK8DZV6N5
- **Principal Investigator:** Elwood Albert Linney
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1993
- **Award amount:** $230,452
- **Award type:** 5
- **Project period:** 1985-02-01 → 1995-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177812

## Citation

> US National Institutes of Health, RePORTER application 3177812, EMBRYONAL CARCINOMA GROWTH AND DIFFERENTIATION (5R01CA039066-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177812. Licensed CC0.

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