# CLONING H-2I AND HLA-D REGION CODING SEQUENCES

> **NIH NIH R01** · STANFORD UNIVERSITY · 1987 · $234,555

## Abstract

The objectives of this research are to understand the relationship between
structure and function of class II major histocompatibility complex (MHC)
molecules and how they regulate the immune response in mouse and man, and
to determine genetic susceptibility to a wide variety of autoimmune and
related diseases.  There are now cDNA and/or genomic clones for murine
A-alpha, A-beta, E-alpha, and E-beta; for HLA-DR-alpha, DR-beta, and
DC-beta; and for genomic sequences in a protochordate tunicate Botryllus,
which are homologous to murine and human class I and class II MHC cDNA
sequences.  These clones will be used:  (1) for synthesis, isolation,
characterization, and sequencing of HLA-DR-beta, DC-beta cDNA clones for
the known DR haplotypes and specifically for HLA-DR and DCl-5 and for
variants subtypes of HLA-DR2,3, and 4, DR-beta, and DC-beta coding
sequences.  Allelic sequence polymorphism will guide synthesis of
oligonucleutide allele-specific probes for typing the known DR and DC
alleles and subtypes of these alleles.  Allelic sequence polymorphism and
allele-specific probes will be used to analyze more precisely the
relationship between class II MHC type and susceptibility to disease in
man; (2) to characterize and sequence genomic and cDNA clones in class I
and class II MHC-like genes and gene products in the protochordate tunicate
Botryllus (sp. Monterey).  These sequences will be used to generate
synthetic peptides and antibodies for use in analyzing expression and
function of these gene products with the aim of understanding their
function in an organism lacking a vertebrate immune response but expressing
MHC-related gene products; (3) to synthesize peptides for allele-specific
sequences of murine and human class II MHC molecules for use as immunogens
to elicit chain-specific and epitope-specific antisera and monoclonal
antibodies for use in functional and biochemical analysis of expression and
function.  Synthetic peptides homologous to similar genes in Botryllus will
also be synthesized and utilized in a similar manner; and (4) to devise
strategies for obtaining high-level expression of cDNA sequences encoding
murine or human alpha and beta class II MHC molecules.  The plan is to
devise cloning strategies to achieve high-level expression of protein
products of parts or all of particular cDNA sequences.  The aim is to
develop the methodology for producing sufficient amounts for biochemical
characterization and collaboration with laboratories expert in X-ray
crystallography.  (CS)

## Key facts

- **NIH application ID:** 3177828
- **Project number:** 5R01CA039069-07
- **Recipient organization:** STANFORD UNIVERSITY
- **SAM.gov UEI:** HJD6G4D6TJY5
- **Principal Investigator:** HUGH O MCDEVITT
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $234,555
- **Award type:** 5
- **Project period:** 1984-09-01 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177828

## Citation

> US National Institutes of Health, RePORTER application 3177828, CLONING H-2I AND HLA-D REGION CODING SEQUENCES (5R01CA039069-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177828. Licensed CC0.

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