# CELLULAR & MOLECULAR CONTROL OF IA ANTIGEN EXPRESSION

> **NIH NIH R01** · UNIVERSITY OF KENTUCKY · 1990 · $130,472

## Abstract

The cellular and genetic mechanisms that control the expression of the
murine Ia antigens will be studied in an effort to better understand the
regulation of the immune response. Since Ia antigen expression can have a
profound effect on the severity and type of immune response generated in
normal and disease situations, understanding the mechanisms of regulation
of Ia expression in different cell types is of central importance. The
specific aims of this proposal are 1) To investigate the intracellular
pathways involved in Ia induction at the membrane, mRNA and
transcriptional levels. This will involve comparison of different methods
of Ia induction and evaluation of the roles of protein kinase C and
cyclic AMP-mediated signal transduction pathways. 2) To further
characterize a newly discovered mechanism of regulation of the E-beta
gene, namely, a block in transcriptional elongation that is regulated by
IFN-gamma. This will involve nuclear run-off analysis of transcription
rates along the E-beta gene in different cell types. 3) Characterize the
sequence involved in the transcriptional blockage by insertion into CAT
vectors specifically designed to evaluate transcriptional termination
sequences. 4) Evaluate the presence of sequence specific DNA binding
proteins that may interact with the region involved in transcriptional
blockage.

## Key facts

- **NIH application ID:** 3177837
- **Project number:** 5R01CA039070-05
- **Recipient organization:** UNIVERSITY OF KENTUCKY
- **SAM.gov UEI:** H1HYA8Z1NTM5
- **Principal Investigator:** Jerold G Woodward
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $130,472
- **Award type:** 5
- **Project period:** 1984-07-01 → 1991-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177837

## Citation

> US National Institutes of Health, RePORTER application 3177837, CELLULAR & MOLECULAR CONTROL OF IA ANTIGEN EXPRESSION (5R01CA039070-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177837. Licensed CC0.

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