# ANALYSIS OF NEONATAL H-2 TOLERANCE

> **NIH NIH R01** · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · 1991 · $218,712

## Abstract

Our long term goal is to understand the cellular and molecular bases of
immunologic tolerance. We have utilized the model system of neonatal
induction of tolerance to H-2 alloantigens and have determined that the
major mechanism responsible for tolerance of class I alloantigens is
clonal elimination. Our recent experimental findings indicate that
tolerance of class II alloantigens is dependent upon the induction of an
active state of specific suppression. From these results we have
formulated the hypothesis upon which the studies outlined in this
proposal are based:

Neonatally-induced tolerance of class II alloantigens is accomplished at
least in part by an active, suppression mechanism that arrest tolerogen-
reactive host T cells at a stage of antigen-induced differentiation prior
to the acquisition of effector function.

We propose two specific aims: 1. To analyze class II specific tolerance
by examining the functional and molecular biologic properties of
tolerogen-reactive cells and to determine the extent to which these
properties mediate suppression and/or are responsible for clonal arrest
prior to acquisition of effector function. 2. To study the resistance of
I-E-negative mice to neonatal tolerance induction with Class I + I-E+
semiallogeneic hematopoietic cells.

Class II-directed T cells dominate the inductive and regulatory
components of immune responses. Therefore, developing an understanding of
the means by which class II-directed T cells are rendered specifically
non-reactive becomes an essential strategy for understanding the primary
means by which the immune system is regulated. The pivotal role of class
II-directed T cells in transplantation immunity, many autoimmune
disorders, and in tumor immunity indicates that our experimental findings
which will illuminate methods for modifying the functional potential of
these cells that will be important in developing future clinical and
therapeutic approaches to these important diseases to man.

## Key facts

- **NIH application ID:** 3177861
- **Project number:** 5R01CA039078-08
- **Recipient organization:** UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
- **SAM.gov UEI:** F8THLJQSAF93
- **Principal Investigator:** J WAYNE STREILEIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $218,712
- **Award type:** 5
- **Project period:** 1983-02-01 → 1994-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3177861

## Citation

> US National Institutes of Health, RePORTER application 3177861, ANALYSIS OF NEONATAL H-2 TOLERANCE (5R01CA039078-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3177861. Licensed CC0.

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