# TYROSINE PHOSPHORYLATION & CONTROL OF CELL GROWTH

> **NIH NIH R01** · ROGER WILLIAMS HOSPITAL · 1988 · $162,599

## Abstract

Protein-tyrosine kinases have been broadly implicated in the
control of both normal and neoplastic cell proliferation.  For
example, the binding of many polypeptide growth factors to their
cellular receptors results in the rapid activation of a receptor-
associated protein-tyrosine kinase that phosphorylates the receptor
itself, as well as a number of cellular proteins.  Furthermore, the
ability of many oncogenic retroviruses to transform cells in
dependent on their encoding and expressing a constitutively active
protein-tyrosine kinase.  As an initial step towards our ultimate
goal of understanding the mechanisms whereby tyrosine
phosphorylation regulates cell growth, we recently produced a
monoclonal antibody that has high affinity and great specificity
for phosphotyrosyl-proteins.  Making extensive use of this
antibody, the proposed studies seek to identify cellular functions
related to cell proliferation that are controlled by protein-
tyrosine kinases and their cellular substrates.  These studies seek
to : (i) identify cellular responses to growth factors and to
oncogenic proteins that are perturbed by antibodies to
phosphotyrosine; (ii) identify cellular functions influenced by
individual phosphotyrosyl-proteins by inserting them into
quiescent, non-transformed cells; (iii) prepare antibodies to three
cellular phosphotyrosyl-proteins that appear to be involved in
tyrosine-kinase responses and to use the antibodies to examine the
expression, distribution and subcellular location of these three
proteins as well as to examine and perturb their functions in
intact cells.  A main approach in all these aims involves the
insertion of antibodies (or phosphotyrosyl proteins) into cells by
osmotic lysis of pinocytic vesicles.

## Key facts

- **NIH application ID:** 3178036
- **Project number:** 2R01CA039235-04A1
- **Recipient organization:** ROGER WILLIAMS HOSPITAL
- **Principal Investigator:** RAYMOND A FRACKELTON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $162,599
- **Award type:** 2
- **Project period:** 1984-08-01 → 1991-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3178036

## Citation

> US National Institutes of Health, RePORTER application 3178036, TYROSINE PHOSPHORYLATION & CONTROL OF CELL GROWTH (2R01CA039235-04A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3178036. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*