# CLONING A MITOGEN-REGULATED PROTEIN

> **NIH NIH R01** · IOWA STATE UNIVERSITY · 1989 · $8,110

## Abstract

Fibroblasts synthesize and secrete a number of new proteins after
stimulation by peptide growth factors such as epidermal growth factor and
fibroblast growth factor.  This response is analogous to the secretion of
lymphokines during elaboration of the immune response.  We believe that as
lymphokines coordinate cellular responses in cell-mediated immunity, these
mitogen-induced proteins secreted by fibroblasts coordinate the growth and
differentiation of solid tissues.  The secreted levels of one
mitogen-induced protein, which we have named "mitogen-regulated protein"
(MRP), appear to be regulated by growth factors at transcription.  Although
growth factors increased the production of MRP, we have observed that
transformation decreases the production of MRP.

We propose to determine whether MRP production and transformation are
consistently inversely correlated.  If so, it could indicate that MRP
production is incompatible with transformation and that MRP might
counteract the transformed state.  We propose to determine what change in
transformed cells results in the decreased production of MRP.  We will also
produce cDNA and genomic clones for MRP.  With these clones we will
determine the effect of growth factors and transformation on the production
of MRP mRNA; the amino acid sequence of MRP; the in vivo distribution of
MRP mRNA; and which geneomic sequences might be responsible for the
regulation of MRP production by growth factors and by transformation.  (N)

## Key facts

- **NIH application ID:** 3178091
- **Project number:** 3R01CA039256-03S1
- **Recipient organization:** IOWA STATE UNIVERSITY
- **SAM.gov UEI:** DQDBM7FGJPC5
- **Principal Investigator:** Marit Nilsen-Hamilton
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $8,110
- **Award type:** 3
- **Project period:** 1985-09-15 → 1989-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3178091

## Citation

> US National Institutes of Health, RePORTER application 3178091, CLONING A MITOGEN-REGULATED PROTEIN (3R01CA039256-03S1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3178091. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*