# RADIOBIOLOGICAL STUDIES OF HUMAN HYBRID CELL LINES

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA-IRVINE · 1987 · $145,510

## Abstract

It is the aim of the proposed research to compare and contrast the
radiobiological properties of the non-tumorigenic and tumorigenic forms of
otherwise well characterized human hybrid cell lines (HeLa x human skin
fibroblasts).  Since the transformation from non-tumorigenic to tumorigenic
forms is associated with certain well-defined changes (e.g. loss of specfic
chromosomes) these cell lines offer a unique system with which to study the
genetic control of radiosensitivity.  In addition radiobiological studies
may indicate oher changes in cellular properties which accompany malignant
transformation (e.g. reduction in DNA repair capability).  Furthermore our
preliminary studies indicate that these human hybrid cells significant
potential for the study of radiaton-induced ongocenic cell transformation.
It is therefore an additional aim of this proposal to develop a methodology
for the quantitative study of radiation-induced oncogenic cell
transformation using hybrid human cells.  A proposed methodology for this
involves detection of malignantly transformed cells using an monoclonal
antibody for a tumor-specific membrane protein and flourescence activated
cell sorting.

## Key facts

- **NIH application ID:** 3178138
- **Project number:** 5R01CA039312-02
- **Recipient organization:** UNIVERSITY OF CALIFORNIA-IRVINE
- **SAM.gov UEI:** MJC5FCYQTPE6
- **Principal Investigator:** JOHN L REDPATH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $145,510
- **Award type:** 5
- **Project period:** 1986-03-01 → 1989-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3178138

## Citation

> US National Institutes of Health, RePORTER application 3178138, RADIOBIOLOGICAL STUDIES OF HUMAN HYBRID CELL LINES (5R01CA039312-02). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3178138. Licensed CC0.

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