# ISOLATION OF AMPLIFIED GENES FROM HUMAN TUMORS

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 1987 · $112,648

## Abstract

The frequent occurrence of amplified genes in human tumors has been
suggested by the observations of double minute chromosomes and
homogeneously staining regions in various types of cancer.  Studies
utilizing cloned probes permitted identification of some of the genes which
are amplified in tumor cells, including several cellular oncogenes.  Other
genes that may be involved at different stages of carcinogenesis and tumor
progression are also likely to be amplified in human malignancies.
Amplification of these unidentified genes can provide a unique opportunity
for their cloning and analysis.  The present proposal is based on the
procedure for detection, comparison, and cloning of amplified genes by
in-gel DNA renaturation, a technique which has been recently developed by
the principal investigator.  This technique detects amplified DNA sequences
in the absence of any preliminary information about their sequence or
location in the genome.  This procedure will be optimized and used to
screen a large number of DNA preparations from various human tumor tissues
and short-term cultues of tumor cells for the presence of amplified DNA
sequences.  The amplified DNA from different tumors will be compared by
in-gel hybridization and the DNA fragments that are amplified in two or
more tumors will be identified.  In addition, the transcribed regions of
the amplified DNA will be detected by hybridization with cDNA probes
corresponding to mRNA from tumor cells.  Those DNA fragments that are
amplified in two or more tumors and/or transcribed in the corresponding
tissues will be cloned.  The resulting clones will be used as probes to
assay their amplification and transcription in various tumors.  Expression
of the cloned gene sequences will be correlated with the tumor type, the
stage of tumor progression, and the history of treatment.  The cloned DNA
sequences will be inserted into expression vectors and expressed in
bacteria and antibodies to the resulting protein products will be raised.
These antibodies will be used for isolation of the proteins encoded by the
amplified genes from tumor cells and for further analysis of the functions
of these proteins.  It is hoped that the proposed experiments will result
in better understanding of the genetic and biochemical mechanisms of tumor
progression, which may lead to the development of new approaches to cancer
diagnostics and treatment.  (I)

## Key facts

- **NIH application ID:** 3178239
- **Project number:** 5R01CA039365-03
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **SAM.gov UEI:** W8XEAJDKMXH3
- **Principal Investigator:** IGOR B RONINSON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $112,648
- **Award type:** 5
- **Project period:** 1985-04-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3178239

## Citation

> US National Institutes of Health, RePORTER application 3178239, ISOLATION OF AMPLIFIED GENES FROM HUMAN TUMORS (5R01CA039365-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3178239. Licensed CC0.

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