# MONOCLONAL ANTIBODIES AGAINST LEUKEMIA

> **NIH NIH R01** · FRED HUTCHINSON CANCER RESEARCH CENTER · 1987 · $154,414

## Abstract

Our objective is to prepare monoclonal antibodies that recognize the
antigens expressed by acute nonlymphocytic leukemic (ANL) colony-forming
cells.  We will then use these antibodies to separate malignant progenitor
cells from those capable of normal hematopoiesis.  Success in these studies
may lead to the development of new methods of treating ANL.

Our approach is based on our previous development of monoclonal antibodies
that recognize normal myeloid stem cells and their progeny at distinct
stages of differentiation.  These monoclonal antibodies were able to
deplete leukemic colony-forming cells from patients with ANL without
completely eliminating normal stem cells.  An important aspect of these
studies was the use of a clonal marker to discern the malignant versus
normal origin of the separated stem cell populations.

In the period of this research we will:  (1) produce monoclonal antibodies
capable of lysing malignant colony-forming cells; (2) examine the cellular
and biochemical specificity of these antibodies; (3) determine the
antigenic phenotype and differentiative capacity of the malignant
colony-forming cells; and (4) use these antibodies to lyse colony-forming
cells present in ANL specimens that express a clonal marker.  Under these
conditions we can test whether the colony-forming cells remaining after
lysis are predominantly derived from the leukemic clone or are of
nonclonal, presumably normal, origin.  This selective depletion of ANL
progenitor cells would provide a rational basis for monoclonal antibody
treatment of ANL.  (MI)

## Key facts

- **NIH application ID:** 3178533
- **Project number:** 5R01CA039492-03
- **Recipient organization:** FRED HUTCHINSON CANCER RESEARCH CENTER
- **SAM.gov UEI:** HMSNCM57QNR5
- **Principal Investigator:** IRWIN D BERNSTEIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $154,414
- **Award type:** 5
- **Project period:** 1985-08-01 → 1988-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3178533

## Citation

> US National Institutes of Health, RePORTER application 3178533, MONOCLONAL ANTIBODIES AGAINST LEUKEMIA (5R01CA039492-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3178533. Licensed CC0.

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