# MAPPING CARCINOGEN BINDING SITES ON GENES

> **NIH NIH R01** · PRINCETON UNIVERSITY · 1987 · $140,428

## Abstract

We describe novel photochemical and enzymatic methods for mapping drug and
carcinogen binding sites on DNA.  We propose to use those methods to
develop rules for predicting benzo(a)pyrene-diol-epoxide (BPDE) binding
sites on eukaryotic genes.  As a class, we propose to use the photochemical
methods as tools to explore the relation between DNA sequence and DNA
structure in the chicken Beta A globin gene, and in the SV40
enhancer/origin of replication.  In a separate set of BPDE binding
experiments we will determine if the c-myc oncogene contains high affinity
carcinogen binding sites and will map the distribution of BPDE molecules
within such regions at one base resolution.  We also describe methods for
mapping BPDE binding to oncogenes in intact nuclei.  The goal of these
experiments is to determine if, in a mouse B cell, the c-myc gene is
involved in chemical carcinogenesis because the gene is sensitive to attack
by an environmental toxin.  We also propose to map BPDE binding sites on
the myc gene in BALB/c-3T3 cells.  The goal of those experiments is to
determine if carcinogen binding to an oncogene shows cell specific
differences.

## Key facts

- **NIH application ID:** 3178599
- **Project number:** 5R01CA039527-03
- **Recipient organization:** PRINCETON UNIVERSITY
- **SAM.gov UEI:** NJ1YPQXQG7U5
- **Principal Investigator:** MICHAEL E. HOGAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $140,428
- **Award type:** 5
- **Project period:** 1985-04-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3178599

## Citation

> US National Institutes of Health, RePORTER application 3178599, MAPPING CARCINOGEN BINDING SITES ON GENES (5R01CA039527-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3178599. Licensed CC0.

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