# VISUALIZATION OF CHROMOSOME EVENTS IN DNA REPAIR

> **NIH NIH R01** · UNIVERSITY OF TX MD ANDERSON CAN CTR · 1987 · $125,683

## Abstract

Agents which induce DNA damage are known to induce mutations, malignant
transformation, and loss of cell viability.  A better understanding of the
carcinogenic process as well as how therapeutic agents used in the
treatment of malignancy induce cell killing requires a knowledge of how the
cell interacts with DNA damage, i.e. DNA repair.  Most previously reported
studies have focused on repair events at the DNA level.  However, little is
known about how the chromatin is modified for DNA excision repair.
Preliminary studies from this laboratory suggest that the technique of
premature chromosome condensation (PCC) can be a poweful tool for probing
chromosome events associated with DNA repair.  The purpose of this project
is to combine the use of the PCC technique, DNA elution, DNA repair
inhibitors, DNA repair deficient cell lines, and immunological techniques
to better understand chromatin aspects of repair.  Four specific aims are
proposed:  (1) Characterize the kinetics of early and late chromatin events
associated with DNA excision repair after ultraviolet (UV) light
irradiation; (2) Determine which types of DNA lesions induce a chromosome
response (i.e. DNA monofunctional adducts, DNA-DNA and DNA-protein
crosslinks, long patch vs short patch repair inducers); (3) Determine
whether specific chromatin events can be inhibited by agents which block
topoisomerase activity, repair synthesis, ligase activity or poly ADP
ribosylation; (4) Explore the use of immunological procedures to specific
factors associated with DNA repair (e.g. DNA polymerase Alpha) to localize
and characterize repair events within chromosomes.

This combination of approaches should yield significant new information
into the chromatin events associated with DNA excision repair and should
shed new light on how cells respond to DNA damage.

## Key facts

- **NIH application ID:** 3178613
- **Project number:** 5R01CA039534-03
- **Recipient organization:** UNIVERSITY OF TX MD ANDERSON CAN CTR
- **SAM.gov UEI:** S3GMKS8ELA16
- **Principal Investigator:** Walter N. HITTELMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $125,683
- **Award type:** 5
- **Project period:** 1985-04-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3178613

## Citation

> US National Institutes of Health, RePORTER application 3178613, VISUALIZATION OF CHROMOSOME EVENTS IN DNA REPAIR (5R01CA039534-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3178613. Licensed CC0.

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