# INDUCTION OF GENE EXPRESSION BY CHEMICAL CARCINOGENS

> **NIH NIH R01** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 1992 · $216,138

## Abstract

The earliest response of most organisms to carcinogenic or toxic
aromatic hydrocarbons is the induction of a set genes which are
involved in the metabolism the carcinogen.  Paradoxically, these
enzymes activate the toxic or carcinogenic potential of the
inducing hydrocarbon.  The cytochromes P1-450 and P3-450 of mouse
or P450c and P450d of rat, are the major induced proteins in this
response in rodents, although induction of other proteins can also
be observed.  The regulation of both cytochromes has been shown to
be transcriptional although the molecular basis of this regulation
has only been studied for P1-450.

The goal of this proposal, is to study the mechanism of P3-450 gene
regulation in a cultured primary rat hepatocyte cell culture system
which we have recently shown can express P450d in response to
hydrocarbon.  We plan to define the cisacting DNA elements required
for MCA induction of the P3-450 gene, as well as to identify
transacting proteins which can interact with the regulator DNA
elements to bring about transcriptional activation.  We further
intend to develop cloned probes for the analysis of other non-P450
genes which are induced by MCA.  With such cloned probes, the
molecular basis of their induction can also be determined.  By
comparing a set of hydrocarbon coregulated genes, the mechanisms
of gene regulation may be determined.

## Key facts

- **NIH application ID:** 3178676
- **Project number:** 5R01CA039553-08
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** Kenneth S Krauter
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $216,138
- **Award type:** 5
- **Project period:** 1985-04-01 → 1993-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3178676

## Citation

> US National Institutes of Health, RePORTER application 3178676, INDUCTION OF GENE EXPRESSION BY CHEMICAL CARCINOGENS (5R01CA039553-08). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3178676. Licensed CC0.

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