# FUNCTIONAL ANALYSIS OF THE ADENO-ASSOCIATED VIRUS GENOME

> **NIH NIH R01** · PRINCETON UNIVERSITY · 1987 · $125,997

## Abstract

Adeno-associated virus (AAV) is a defective parvovirus which requires
helper function from one of two DNA tumor viruses, adenovirus or herpes
simplex virus.  AAV not only utilizes gene products from these tumor
viruses, but it interferes with their growth and with oncogenicity of cells
transformed by these viruses.  Finally, AAV is able to integrate into host
cell DNA in the absence of helper virus and maintain a latent infection.
Thus, AAV is of considerable interest both in terms of its ability to
maintain itself in host cells and its interaction with its helper viruses.
The goal of the research proposed here is to describe these interactions in
molecular terms.  Specifically, we will:  (1) utilize existing adenovirus
deletion mutants to more precisely define helper virus functions required
for efficient growth of AAV in cultured cells; (2) define the site(s) of
interference by AAV with adenovirus growth; (3) study the effects of
deletions prepared in an infectious recombinant plasmid on AAV growth; (4)
devise a method by which to propagate AAV mutants defective for growth in
the presence of wild-type helper virus.

## Key facts

- **NIH application ID:** 3178784
- **Project number:** 5R01CA039606-03
- **Recipient organization:** PRINCETON UNIVERSITY
- **SAM.gov UEI:** NJ1YPQXQG7U5
- **Principal Investigator:** THOMAS E SHENK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $125,997
- **Award type:** 5
- **Project period:** 1985-04-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3178784

## Citation

> US National Institutes of Health, RePORTER application 3178784, FUNCTIONAL ANALYSIS OF THE ADENO-ASSOCIATED VIRUS GENOME (5R01CA039606-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3178784. Licensed CC0.

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