# MECHANISMS OF TUMOR CELL INVASION AND EXTRAVASATION

> **NIH NIH R01** · MONTANA STATE UNIVERSITY - BOZEMAN · 1987 · $109,571

## Abstract

This research seeks to improve our understanding of the basic mechanisms of
tumor cell extravasation.  Using capillary endothelial cell monolayers and
primary tumor cells in vitro, we will document, in detail, the nature of
the cell surface components involved in tumor:endothelial attachment.
Those monoclonal antibodies reacting with endothelial cell surface
components, which we have found to block tumor:endothelial attachment in
vitro will be studied for the ability to block lung colonization.  The
tumor specificity (or lack thereof) of the blocking effect will be
determined as well as the nature of the cell surface components involved.
The mechanisms whereby neutrophils or activated macrophages can induce
increased tumor:endothelial attachment, tumor localization in the lung and
lung colonization will be investigated.  The biochemical nature of actual
basement membrane matrix degradation by tumor cells will be examined using
isolated lens capsule analyzed in various ways including electrophoresis
and immunoblot techniques, and the relationship of matrix degradation to
basement membrane invasion directly determined using the same matrix
substrate.  These studies will include the use of tumor variants selected
for the ability to invade across the lens capsule basement membrane, broad
spectrum antiproteases and mixtures of complementary protease inhibitors.
Possible contributions of various classes of leukocytes to the invasive
steps of tumor cell extravasation will be examined.

In addition to the intrinsic value of these studies to the understanding of
tumor pathogenesis, these studies will provide information relevant to the
design of antimetastatic therapies which seek to block extravasation
forcing tumor emboli to remain in the circulation . . . . a naturally
adverse environment for them and one which is very amenable to therapeutic
manipulation.

## Key facts

- **NIH application ID:** 3178793
- **Project number:** 5R01CA039611-04
- **Recipient organization:** MONTANA STATE UNIVERSITY - BOZEMAN
- **SAM.gov UEI:** EJ3UF7TK8RT5
- **Principal Investigator:** JEAN Rosalind STARKEY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $109,571
- **Award type:** 5
- **Project period:** 1982-04-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3178793

## Citation

> US National Institutes of Health, RePORTER application 3178793, MECHANISMS OF TUMOR CELL INVASION AND EXTRAVASATION (5R01CA039611-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3178793. Licensed CC0.

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