# TRANSFORMATION BY RESTRICTION FRAGMENTS OF HSV DNA

> **NIH NIH R01** · UNIVERSITY OF MARYLAND BALTIMORE · 1987 · $128,242

## Abstract

Our recent studies on the mechanism of HSV-2 induced transformation have
shown that: (i) neoplastic transformation of normal diploid cells is
mediated by the Bg1 II C fragment of HSV-2 DNA (0.416-0.580 map units),
(ii) the transformation process involves at least two steps: cellular
immortalization mediated by sequences within 0.410-0.525 map units, and the
acquisition of neoplastic potential that requires sequences within
0.525-0.580 map units on the HSV-2 genome (presumptive neoplastic
sequences), and (iii) the expression of a viral protein designated ICP 10
that is encoded within the presumptive neoplastic sequences correlates with
the acquisition of anchorage independent growth and tumorigenic potential
by HSV-2 transformed cells.  The studies proposed in this application are
designed to obtain a better understanding of the role played by the
presumptive neoplastic sequences and the proteins that they encode,
primarily ICP10, in neoplastic transformation.  The specific aims are:
(i)\to determine and compare the transforming activities of the
subfragments of the presumed neoplastic sequences, in transforming
activities of the subfragments of the presumed neoplastic sequences, in
various combinations with immortalizing sequences and the sequences
homologous to the various combinations with immortalizing sequences and the
sequences homologous to the mammalian cell genome, (ii) to determine and
compare the transforming activities (at 34 and 39 C) of the tsA8 mutant (in
130K and ICP 10 proteins) before and after marker rescue of the
conditionally lethal function (in 130K), (iii) to inhibit ICP 10 synthesis
with an oligonucleoside methylphosphonate complementary to the translation
initiation region of the ICP 10 mRNA and determine the effect on
transformation, (iv) to define and identify DNA sequences homologous to the
presumptive neoplastic sequences in the transformed lines and their
potential interaction with hamster cell DNA sequences and (v) to identify
viral proteins in the transformed cells with particular emphasis on ICP 10,
a protein that is encoded by the presumptive neoplastic sequences.  These
studies should provide a better understanding of the mechanism of HSV-2
induced neoplastic transformation.

## Key facts

- **NIH application ID:** 3179019
- **Project number:** 5R01CA039691-03
- **Recipient organization:** UNIVERSITY OF MARYLAND BALTIMORE
- **SAM.gov UEI:** Z9CRZKD42ZT1
- **Principal Investigator:** Laure Aurelian
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $128,242
- **Award type:** 5
- **Project period:** 1985-04-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179019

## Citation

> US National Institutes of Health, RePORTER application 3179019, TRANSFORMATION BY RESTRICTION FRAGMENTS OF HSV DNA (5R01CA039691-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179019. Licensed CC0.

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