# MECHANISM OF LYMPHOCYTE MITOGENESIS

> **NIH NIH R01** · UNIVERSITY OF MINNESOTA · 1987 · $133,565

## Abstract

The general goal of this project is to define the mechanism of mitogenesis
in nontransformed human lymphocytes and the relationship of transforming
gene products to this process.  The work focuses on the transition of
resting, GO phase lymphocytes from peripheral blood into the cell cycle in
response to three required signals:  (1) mitogenic lectin or antigen; (2)
serum factors; and (3) interactions between cell types.  In transformed
cells control of proliferation by these signals is relaxed.  The aims
concentrate on defining the role of a limited number of intracellular
proteins that are preferentially expressed or synthesized in response to
these signals.  The hypothesis is that these proteins are part of the
intracellular signal transduction pathway leading to cell proliferaton.
The aims are:  (1) determination of the expression and synthesis of
proteins associated with proliferative response in mononuclear cell subsets
and transformed cells; (2) determination of kinetics of production and
degradation of response-associated proteins and relationship to known
transforming gene products; and (3) determination of association between
signal type and expression of proteins.  The methods are designed to
dissect the effects of each signal type on protein expression and resulting
proliferation, aggregation, or adhesion of the cells.  The methods
include:  separation of cell types by surface markers and density,
determination of patterns of protein expression and synthesis by one-\and
two-dimensional eletrophoresis, kinetic assays for proliferation, binding
of antibodies to known oncogene products, and tentative steps toward
physical isolation of response-associated proteins.  A major justificstion
for working with this complex cell system is that it represents a normal
rather than an imposed quiescent state in cells that can be moved partway
along the signal transduction pathways by separate signals.  The results
are expected to contribute significantly to the understanding and ultimate
control of human malignancy.  (LB)

## Key facts

- **NIH application ID:** 3179027
- **Project number:** 5R01CA039692-03
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **SAM.gov UEI:** KABJZBBJ4B54
- **Principal Investigator:** JAMES J O'LEARY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $133,565
- **Award type:** 5
- **Project period:** 1985-09-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179027

## Citation

> US National Institutes of Health, RePORTER application 3179027, MECHANISM OF LYMPHOCYTE MITOGENESIS (5R01CA039692-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179027. Licensed CC0.

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