# REGULATION OF ADENYLATE CYCLASE UPON TRANSFORMATION

> **NIH NIH R01** · UNIVERSITY OF PENNSYLVANIA · 1987 · $96,785

## Abstract

The objective of the proposed research is to determine the extent to which
modifications of the guanine nucleotide-binding proteins Gs and Gi
participate in the regulation of adenylate cyclase coincident with
malignant transformation.  Transformation of various cells by Rous and
Kirsten sarcoma viruses will be empoloyed as models for investigation.  An
initial aim of the proposed research is the analysis of Gs and Gi
function in relation to transformation.  The ability of Gs and Gi to
mediate activation and inhibition of adenylate cyclase in native plasma
membranes and in reconstituted systems will be investigated.  A subsequent
aim is the development of antibodies specific for subunits of Gs and
Gi.  Antibodies will be elicited by immunization with conjugated
synthetic peptides that correspond to hydrophilic regions of these
proteins.  Serum titer and specificity will be monitored by solid-phase
techniques and procedures of immunoprecipitation.  The third aim is the
assessment of regulatory events that may impinge upon Gs and Gi in
relation to transformation.  Events to be investigated include alterations
in level of Gs and Gi, alterations in relative levels of isozymic forms
of these proteins, post-translational modification, and physical
interactions with other proteins.  This aim will be achieved by employment
of radiolabel and immunological procedures.  Alterations in basal
activities of adenylate cyclase, and in the responsiveness of this enzyme
to extracellular agents, have been suggested as participating in
transformation, metastasis, differentiation, and progression of the cell
cycle.  The ultimate objective of the proposed research is to contribute to
the understanding of the relationship between these alterations and the
general phenomenon of cell proliferation.  (N)

## Key facts

- **NIH application ID:** 3179068
- **Project number:** 5R01CA039712-03
- **Recipient organization:** UNIVERSITY OF PENNSYLVANIA
- **SAM.gov UEI:** GM1XX56LEP58
- **Principal Investigator:** David Randell Manning
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $96,785
- **Award type:** 5
- **Project period:** 1985-04-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179068

## Citation

> US National Institutes of Health, RePORTER application 3179068, REGULATION OF ADENYLATE CYCLASE UPON TRANSFORMATION (5R01CA039712-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179068. Licensed CC0.

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