# ASSAYS FOR AND MECHANISMS OF ACTION OF TUMOR PROMOTERS

> **NIH NIH R01** · UNIVERSITY OF HAWAII AT MANOA · 1987 · $207,557

## Abstract

The ability of neoplastic cells to initiate DNA synthesis and proliferate
in Ca2+-deficient medium distinguishes them from their non-neoplastic
counterparts.  A clue to understanding this alteration is the fact that
tumor promoting agents enable non-neoplastic rat liver epithelial cells to
initiate DNA synthesis and proliferate in Ca2+-deficient medium like their
neoplastic counterparts.  It follows that uncovering the mechanisms
responsible for this phenomenon should not only enhance our knowledge of
the neoplastic process but also provide opportunities to modulate the tumor
promotion process.  These are our long range objectives.  This proposal
will test the hypothesis that tumor promoters in general stimulate
Ca2+-deprived rat liver cells to initiate DNA synthesis through a mechanism
involving Ca2+-calmodulin, cyclic AMP, cyclic AMP-dependent protein kinases
and Ca2+/phospholipid-dependent protein kinase activity.  The cell surface
and intracellular phosphoprotein substrates will be identified and their
requirement for DNA-synthetic activity and tumor promotion determined.

## Key facts

- **NIH application ID:** 3179135
- **Project number:** 5R01CA039745-03
- **Recipient organization:** UNIVERSITY OF HAWAII AT MANOA
- **SAM.gov UEI:** NSCKLFSSABF2
- **Principal Investigator:** ALTON L BOYNTON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $207,557
- **Award type:** 5
- **Project period:** 1985-08-01 → 1988-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179135

## Citation

> US National Institutes of Health, RePORTER application 3179135, ASSAYS FOR AND MECHANISMS OF ACTION OF TUMOR PROMOTERS (5R01CA039745-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179135. Licensed CC0.

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