# GENE EXPRESSION IN LIVER CARCINOGENESIS

> **NIH NIH R01** · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · 1987 · $93,851

## Abstract

The expression of specific genes which may be involved in the neoplastic
transformation of rat liver during chemical carcinogenesis will be
studied.  These genes will be identified in two ways:  1) known oncogenes
will be tested for their expression in rat liver tumors using hybridization
of liver mRNA to (32-P) labelled probes (RNA dot-blots).  Because increased
levels of oncogene expression are frequently correlated with the neoplastic
state in other cancers, there is a good possibility that the levels of
oncogene transcripts will be elevated in rat liver tumors; 2) other tumor
specific transcripts will be identified by constructing a cDNA library made
from rat liver tumor mRNA, and then testing the hybridization of individual
cDNA clones to (32-P) labelled mRNA from tumor and from normal liver.  With
these two approaches, mRNA sequences whose amounts are elevated in liver
carcinomas compared to normal liver will be identified.  If previously
unknown mRNA's are found, the proteins which they encode will be identified
by a computer analysis of their cDNA sequences.

Tumor specific cDNA clones will be used to study two important problems in
carcinomas compared to normal liver will be identified.  If previously
unknown mRNA's are found, the proteins which they encode will be identified
by a computer analysis of their cDNA sequences.

Tumor specific cDNA clones will be used to study two important problems in
carcinogenesis:  1) in situ hybridization of tissue sections and cell
fractionation will be used to find which cell types are transcribing tumor
specific genes during carcinogenesis, in hopes of identifying premalignant
cell populations which ultimately give rise to tumors; 2) the pattern of
expression of tumor specific genes during tumnor induction with different
carcinogens will be studied.  Comparisons among different carcinogens may
determine whether they act by common or by distinct mechanisms.

## Key facts

- **NIH application ID:** 3179198
- **Project number:** 5R01CA039792-03
- **Recipient organization:** UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
- **SAM.gov UEI:** ZUFBNVZ587D4
- **Principal Investigator:** BRIAN J KNOLL
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $93,851
- **Award type:** 5
- **Project period:** 1985-04-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179198

## Citation

> US National Institutes of Health, RePORTER application 3179198, GENE EXPRESSION IN LIVER CARCINOGENESIS (5R01CA039792-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179198. Licensed CC0.

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