# TRANSFORMATION BY HUMAN RAS ONCOGENES

> **NIH NIH R01** · COLD SPRING HARBOR LABORATORY · 1987 · $231,508

## Abstract

The function of the ras family of oncogene proteins will be investigated
utilizing both living cell systems and in vitro biochemical approaches.
The human H-ras proto-\and oncogene proteins have been produced in an E.
coli expression system, and the proteins have been purified to homogeneity
and characterized as "native" entities.  We propose to use these intact
proteins as antigens for the production of a bank of mouse-derived
monoclonal antibodies, which will be subsequently employed in both
biochemical experiments and microinjection experiments with normal and
transformed cells.  As we have recently shown, microinjection of the human
H-ras  oncogene protein, but not the proto-oncogene protein, into quiescent
normal cells results in rapid morphological changes followed by DNA
synthesis and proliferation.  We wish to follow up these initial
experiments by examining this unique assay of the transformation process
induced by human oncogene proteins in great detail.  And, through the use
of the purified human H-ras proteins, we intend to look at the interactions
of other cellular proteins with the ras proteins in vitro.  (X)

## Key facts

- **NIH application ID:** 3179208
- **Project number:** 5R01CA039811-03
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **SAM.gov UEI:** GV31TMFLPY88
- **Principal Investigator:** JAMES R FERAMISCO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $231,508
- **Award type:** 5
- **Project period:** 1985-04-01 → 1988-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179208

## Citation

> US National Institutes of Health, RePORTER application 3179208, TRANSFORMATION BY HUMAN RAS ONCOGENES (5R01CA039811-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179208. Licensed CC0.

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