# MECHANISM OF GAMMA INTERFERON REGULATION OF IA ANTIGENS

> **NIH NIH R01** · INDIANA UNIVERSITY INDIANAPOLIS · 1987 · $113,150

## Abstract

Recent work from several laboratories has shown that murine gamma
interferon induces the biosynthesis and cell surface expression of
immune-associated (Ia) glycoprotein determinants on both normal and
neoplastic mouse mononuclear phagocyte lineage cells.  Experiments carried
out by the principal investigator demonstrate that gamma interferon
activates a murine macrophage tumor cell line, (P-388.D1), to release a
second, noninterferon factor activity which, in turn, stimulates Ia
expression on mouse macrophage tumor cells.  The major goal of this
research proposal is to test the hypothesis that gamma interferon regulates
the positive expression of macrophage Ia determinants by stimulating the
induction of a second group of Ia-inducing factors (IaIF) by the macrophage
which subsequently control the cell surface expression of individual Ia
subregion determinants.  Thus, a variety of murine macrophage tumor cell
lines and normal mononuclear phagocyte subpopulations will be cultured in
vitro using different Ia induction procedures, and conditioned medium from
these cells will be tested against neoplastic and normal Ia negative
macrophage target cells for the ability to induce Ia expression.
Quantitation of Ia induction will be carried out using direct and indirect
immunofluorescence with anti-Ia monoclonal antibodies and flow cytometry
analysis (FcM).  Where active IaIF supernatants are found, they will be
examined using standard biochemical analysis techniques in an effort to
characterize the material and show if there are multiple IaIF activities
which control the expression of discrete Ia subregion glycoproteins.
Normal or neoplastic macrophages which are induced by IaIF activity for one
or more Ia subregion antigens (i.e., I-A, I-E/C, or I-J), will be studied
for functional activity as antigen-presenting cells in T-cell proliferation
and activation assays using MHC-restricted, antigen-specific, continuous
helper T-cell lines, or T hybridomas as effector cells.  I-J-inducible
macrophages will be tested as accessory cells in the induction of a primary
in vitro burro red blood cell IgM response, and as antigen-presenting cells
in the activation of third-order suppressor T cells (Ts 3) in the
azobenzenearsonate (ABA) system.  This study will provide basic information
describing the mechanism by which gamma interferon may regulate the cell
surface expression of individual Ia subregion antigens on phenotypically
and functionally unique subsets of mononuclear phagocytes.  (SR)

## Key facts

- **NIH application ID:** 3179285
- **Project number:** 5R01CA039925-03
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **SAM.gov UEI:** SHHBRBAPSM35
- **Principal Investigator:** EDWIN B WALKER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $113,150
- **Award type:** 5
- **Project period:** 1985-01-01 → 1988-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179285

## Citation

> US National Institutes of Health, RePORTER application 3179285, MECHANISM OF GAMMA INTERFERON REGULATION OF IA ANTIGENS (5R01CA039925-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179285. Licensed CC0.

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