# IMMUNOLOGICAL STUDIES OF MEMBRANE ANTIGENS

> **NIH NIH R01** · DUKE UNIVERSITY · 1988 · $153,253

## Abstract

The long-term objective of these studies is to define selected antigens of
human cells using antibodies produced in non-human primates.  The
immunological perspective of the non-human primates should allow the
recognition of epitopes on these antigens which may be difficult to be
detected by lower mammalian species.  The specific aims for this renewal
period are to: (1) establish the optimal conditions and method for
production of chimpanzee monoclonal antibodies to selected human normal and
malignant cell antigens; (2) establish the optimal conditions and methods
for the in vitro transfection or infection of chimpanzee cells with
oncogenes or human tumor DNA; and (3) from aim 2, establish the
immunogenicity of tumorigenicity of chimpanzee cells that show evidence of
gene insertion or changes in vitro growth characteristics.

Specifically, aim 1 will evaluate various methods of chimpanzee B-cell
fusion such as electrofusion and PEG, varying the conditions for fusion and
growth as well as the parental human and murine myeloma lines to be used as
fusion partners.  In particular, aim 2 will evaluate various in vitro
methods of gene insertion including transfection and infection of
chimpanzee fibroblast, epithelial cells, or lymphocytes.  Aim 3 will
utilize standard injection and serological procedures adapted in this
laboratory to induce and measure the autologous immune response in
chimpanzees to human gene insertion, their in vitro growth properties, and
the nature of the insertion technique.  (CS)

## Key facts

- **NIH application ID:** 3179480
- **Project number:** 5R01CA040044-25
- **Recipient organization:** DUKE UNIVERSITY
- **SAM.gov UEI:** TP7EK8DZV6N5
- **Principal Investigator:** RICHARD S METZGAR
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $153,253
- **Award type:** 5
- **Project period:** 1975-05-01 → 1990-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179480

## Citation

> US National Institutes of Health, RePORTER application 3179480, IMMUNOLOGICAL STUDIES OF MEMBRANE ANTIGENS (5R01CA040044-25). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179480. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*