# DNA SEQUENCE CHANGES IN MUTATED MAMMALIAN CELLS

> **NIH NIH R01** · YALE UNIVERSITY · 1989 · $40,774

## Abstract

It is proposed to determine DNA sequence changes induced by specific
mutagens in the E. coli gpt gene, of which a single copy has been stably
integrated into the genome of a line of CHO cells.  The product of the gpt
gene in this cell line, AS52, is functionally equivalent to the product of
the mammalian hprt gene, which has been deleted.  Thus, forward and back
mutations in gpt can be assayed in AS52 cells by the well-tested methods
developed to assay mutations in hprt in CHO cells.  The gpt gene is only
456 base pairs in size, so it is much easier to sequence than the hprt gene
(about 32 kilobases).  An active Apr gene is adjacent to the gpt gene,
which will greatly facilitate recovery of the mutated (inactive) gpt gene
from the AS52 genome by plasmid rescue.  Finally, it is possible to
determine sequence changes induced by the same agents in the same gene in
E. coli cells, and obtain comparative data in both mammalian and bacterial
cells.  Mutagenesis by ultraviolet light and by spontaneous processes will
be studied first.  The next agents will probably be prototype compounds:
ICR-191, an acridine derivative which acts as an intercalator in bacterial
cells; MNNG (N-methyl-N1-nitro-N-nitrosoguanidine; an alkylating agent;
bromouracil, a base analog; EMS (ethylmethane sulfonate); etc.

## Key facts

- **NIH application ID:** 3179840
- **Project number:** 3R01CA040195-03S1
- **Recipient organization:** YALE UNIVERSITY
- **SAM.gov UEI:** FL6GV84CKN57
- **Principal Investigator:** FRANKLIN HUTCHINSON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $40,774
- **Award type:** 3
- **Project period:** 1985-08-01 → 1989-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179840

## Citation

> US National Institutes of Health, RePORTER application 3179840, DNA SEQUENCE CHANGES IN MUTATED MAMMALIAN CELLS (3R01CA040195-03S1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179840. Licensed CC0.

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