# FUNCTIONAL CHARACTERIZATION OF ISOLATED PDGF RECEPTOR

> **NIH NIH R01** · HARVARD MEDICAL SCHOOL · 1987 · $152,606

## Abstract

Platelet-derived growth factor (PDGF) is a potent mitogen for connective
tissue cells, such as human skin fibroblasts.  The plasma membranes of
these cells contain specific receptor proteins for PDGF.  When PDGF binds
to the receptor, a protein kinase activity is activated which
phosphorylates the receptor at tyrosine and serine amino acid resides.  The
first aim of this proposal is to solubilize the PDGF receptor from human
fibroblasts and characterize its binding and kinase activities in
solution.  The second aim is to use the technique of peptide mapping to
determine which subdomains of the molecule contain the functional sites.
These studies should lead to a clearer picture of the receptor and provide
insight into the mechanism by which PDGF and it receptor stimulate cell
proliferation.  (J)

## Key facts

- **NIH application ID:** 3179874
- **Project number:** 5R01CA040214-03
- **Recipient organization:** HARVARD MEDICAL SCHOOL
- **SAM.gov UEI:** JDLVAVGYJQ21
- **Principal Investigator:** ALBERT J OWEN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $152,606
- **Award type:** 5
- **Project period:** 1985-08-01 → 1988-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179874

## Citation

> US National Institutes of Health, RePORTER application 3179874, FUNCTIONAL CHARACTERIZATION OF ISOLATED PDGF RECEPTOR (5R01CA040214-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179874. Licensed CC0.

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