# CHARACTERIZATION OF HUMAN B-CELL ACTIVATION ANTIGENS

> **NIH NIH R01** · DANA-FARBER CANCER INST · 1987 · $164,049

## Abstract

The overall aim of the project is the characterization of functionally
important cell surface antigens (Ags) expressed on activated human B
lymphocytes.  The research project's interest in these molecules is based
on the notion that these Ags are prime candidates for those cell surface
structures which control B-cell activation, proliferation, and
differentiation.  In the proposal, it is planned to undertake a detailed
analysis of B-cell activation Ags with specific attention to:  (1) the
stimuli that induce their expresssion; (2) their relationship to other
B-cell Ags expressed on both resting and activated B cells; (3) their
relationship to growth factor and/or accessory cells that are involved with
activation, proliferation, and differentiation of B cells; and (4) the
expression of the Ags on neoplastic B cells and their possible role in the
induction and control of B-cell neoplasia.  To accomplish this, research is
planned to develop methods to isolate and activate human B cells in vitro
and develop culture systems which will allow delineation of the cell
surface antigenic changes which accompany activation, proliferation, and
differentiation.  Using the expression of these cell surface Ags to
identify the stages of B-cell activation, the attempt will be made to
isolate unique populations of activated B cells and study their response to
inducers of activation and differentiation.  The goals are to:  (1)
identify and characterize those cell surface molecules which are important
in the induction of these processes; (2) study previously known B-cell
activation Ags; (3) develop monoclonal antibodies to new activation Ags
with the intention of relating these structures to the stages of B-cell
activation and differentiation as well as defining the functional
importance of each of these molecules; (4) examine the expression of B-cell
activation Ags on B-cell leukemias and lymphomas; and (5) attempt to
determine whether there is a correlation between antigen expression on
neoplastic B cells and whether they have both the ability to respond to
physiologic triggers of activation in vitro as well as the clinical course
of the disease.  (AG)

## Key facts

- **NIH application ID:** 3179880
- **Project number:** 5R01CA040216-03
- **Recipient organization:** DANA-FARBER CANCER INST
- **SAM.gov UEI:** DPMGH9MG1X67
- **Principal Investigator:** Lee Marshall Nadler
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $164,049
- **Award type:** 5
- **Project period:** 1985-08-01 → 1988-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179880

## Citation

> US National Institutes of Health, RePORTER application 3179880, CHARACTERIZATION OF HUMAN B-CELL ACTIVATION ANTIGENS (5R01CA040216-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179880. Licensed CC0.

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