# ULTRASOUND AND MALIGNANT TRANSFORMATION IN VITRO

> **NIH NIH R01** · UNIVERSITY OF MARYLAND BALTIMORE · 1987 · $94,793

## Abstract

We propose to assess the oncogenic risk of ultrasound, using the
C3H/10T1/2 in vitro assay for malignant transformation.  This
assay is a more direct and quantitative measure of oncogenic risk
than are assays of mutation or chromosomal alterations.  Pulsed
ultrasonic beams configured to simulate diagnostic conditions will
be used to irradiate cells alone, in combination with carcinogenic
treatment, or followed by post-treatment culturing in 0.1 /g/ml of
12-0-tetradecanoyl-phorbol-13-acetate (TPA) in order to
investigate the possible oncogenic interaction of co-stressors and
tumor promotors with ultrasound.

In examining the effect of ultrasound alone, experiments will be
designed to detect a malignant transformation rate equivalent to
or greater than the response of 1 Gy of 250 kVp X-rays or to
about 2 /g/ml of benzo(a)pyrene, with 95% statistical confidence
above the spontaneous transformation rate.  In examining the
effect of ultrasound as a co-stressor, experiments will be designed
to detect a two-fold enhancement of transformation by ultrasound
with 95% statistical confidence.  Cells will be insonated in
suspension in a transmission chamber at 37 degrees C using
uniform, minimally focussed beam profiles, with insonation
parameters simulating the upper range of pressure amplitudes
from clinical ultrasonic imaging and doppler systems.  Emphasis
will be placed on providing replicable, well-characterized
ultrasound delivery and bioassay.

## Key facts

- **NIH application ID:** 3179890
- **Project number:** 2R01CA040223-03
- **Recipient organization:** UNIVERSITY OF MARYLAND BALTIMORE
- **SAM.gov UEI:** Z9CRZKD42ZT1
- **Principal Investigator:** GEORGE H HARRISON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $94,793
- **Award type:** 2
- **Project period:** 1985-07-01 → 1990-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179890

## Citation

> US National Institutes of Health, RePORTER application 3179890, ULTRASOUND AND MALIGNANT TRANSFORMATION IN VITRO (2R01CA040223-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179890. Licensed CC0.

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