# MOLECULAR MECHANISMS OF HYDROCARBON DNA INTERACTIONS

> **NIH NIH R01** · PURDUE UNIVERSITY · 1988 · $144,063

## Abstract

The overall objective of this research is to determine how carcinogenic
polycyclic aromatic hydrocarbons bind to DNA in cell cultures from
different species and the relationship of this binding to the induction of
biological effects.  This will be accomplished by analyzing the
hydrocarbon-DNA interaction products formed in cell cultures from several
species by both immobilized boronate chromatography and high-pressure
liquid chromatography to determine the nature of the adducts formed and the
relative persistence of specific adducts.  Identification of the structures
of the hydrocarbon-DNA adducts will be carried out directly by the
application of mass spectrometric techniques as well as by radioisotope
studies involving hydrolysis and chromatography.  Based upon the types of
hydrocarbon-DNA adducts formed, cells from various species will be selected
for use as activators in mammalian cell-mediated mutation assays.  The V79
target cells will be separated from the activator cells by an
immunoseparation procedure and the hydrocarbon-DNA adducts formed in the
target cells will be analyzed.  The relationship of the formation of
specific hydrocarbon-DNA adducts to the induction of mutation and
cytotoxicity will be investigated.  The carcinogenic hydrocarbons to be
studied include both the unsubstitutedd hydrocarbon benzo(a)pyrene and the
methyl-substituted, extremely potent carcinogen 7,
12-dimethylbenz(a)anthracene.  At present very little is known about the
role of specific 7,12-dimethylbenz(a)anthracene-DNA adducts in the
induction of biological effects.  The proposed studies will determine how
these hydrocarbons interact with DNA, how these interactions vary in cells
from different species and how specific types of hydrocarbon-DNA
interactions are related to the induction of biological effects.  This
information will help to establish which types of hydrocarbon-DNA
interactions are important for carcinogenesis and what characteristics
would make a cell, tissue or species susceptible to these types of
hydrocarbon-DNA interactions.

## Key facts

- **NIH application ID:** 3179910
- **Project number:** 5R01CA040228-04
- **Recipient organization:** PURDUE UNIVERSITY
- **SAM.gov UEI:** YRXVL4JYCEF5
- **Principal Investigator:** Donald E. BERGSTROM
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $144,063
- **Award type:** 5
- **Project period:** 1985-07-01 → 1990-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179910

## Citation

> US National Institutes of Health, RePORTER application 3179910, MOLECULAR MECHANISMS OF HYDROCARBON DNA INTERACTIONS (5R01CA040228-04). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179910. Licensed CC0.

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