# MOLECULAR MECHANISMS OF HYDROCARBON-DNA INTERACTIONS

> **NIH NIH R01** · PURDUE UNIVERSITY · 1991 · $178,701

## Abstract

DESCRIPTION:  (Adapted from the applicant's abstract):  The proposed
studies with various mammalian activator cells will relate the metabolic
activation and DNA binding of benzo(a)pyrene [B(a)P] and
dimethylbenza(a)anthracene (DMBA).  The specific aims are:  1) To continue
studies of the role of specific cytochrome P-450 induction in the binding
of B(a)P and DMBA to DNA in mammalian cells through the use of antibodies
against specific cytochrome P-450 isozymes; 2) To continue the development
of a lambda35S-ATP postlabeling procedure to allow detection of unlabeled
hydrocarbon-DNA adducts at low levels with the advantages of analysis at
the mononucleotide level by HPLC and the use of 35S rather than 32P.  This
assay will be used to facilitate studies in activator and target cells
under conditions of mutation assays; 3) To investigate the binding of DMBA
and B(a)P to specific regions of chromatin and DNA in cells from various
species through the use of immunoaffinity chromatography on columns
prepared with antibodies against DMBADE-DNA adducts and B(a)P-DNA adducts;
4) To determine the role of base sequence in the binding of DMBA and B(a)P
to DNA through the use of defined sequence oligonucleotides produced by
PCR; 5) To determine the relationship of DMBA and B(a)P adduct formation to
the formation of oxidative damage as measured by formation of 8-hydroxy-dG
in DNA in mammalian cells; 6) To relate the binding of B(a)P and DMBA to
DNA to produce specific adducts or to specific regions of DNA and chromatin
to mutation induction in cell-mediated V79 cell assays.

## Key facts

- **NIH application ID:** 3179912
- **Project number:** 5R01CA040228-07
- **Recipient organization:** PURDUE UNIVERSITY
- **SAM.gov UEI:** YRXVL4JYCEF5
- **Principal Investigator:** Donald E. BERGSTROM
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $178,701
- **Award type:** 5
- **Project period:** 1985-07-01 → 1995-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179912

## Citation

> US National Institutes of Health, RePORTER application 3179912, MOLECULAR MECHANISMS OF HYDROCARBON-DNA INTERACTIONS (5R01CA040228-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179912. Licensed CC0.

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