# RADIATION-INDUCED G2-ARREST AND PROTEIN SYNTHESIS

> **NIH NIH R01** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 1988 · $150,013

## Abstract

Protein synthesis is required for a cell to recover from radiation-
induced G2-arrest.  It is the purpose of this investigation to
determine the nature and functions of the protein(s) involved.
Two lines of inquiry will be followed.  The first is prompted by the
observation that an arrested cell can be brought into a mitosis-
like state by fusion with a mitotic cell.  This process is believed
to be mediated by the donation of a group of polypeptides termed
Maturation Promoting Factors (MPF), thus it is suggested that
recovery from radiation-induced G2-arrest may require MPF
synthesis.  The second approach is prompted by the observation
that arrested cells may be brought into mitosis without protein
synthesis by treatment with caffeine and cycloheximide.  This
suggests that the components of the progression "machinery" are
actually intact in arrested cells (including MPF), but are
inactivated.  The proteins normally required for recovery must
then be produced for some other activity, not directly related to
progression, and probably unique to, overly abundant in, or
characterized by enhanced synthesis in irradiated cells compared
to age-equivalent controls.

MPF will be isolated from arrested cells obtained by centrifugal
elutriation and assayed for activity in Xenopus laevis oocytes.
Protein synthesis in arrested cells will be monitored by
incorporation of isotope-labelled amino acids, then the cells
retrieved from arrest as a discrete population by caffeine
treatment and mitotic cell selection.  Specific polypeptides will
be identified by 2-D gel electrophoresis.

The work proposed is intended to determine the requirements for
progression past the X-ray transition point.  Such information may
help us to understand why cell sensitivity to killing by irradiation
increases so markedly at the X-ray transition point and may
provide the means to manipulate both cell progression and cell
radiosensitivity, both factors of importance to the treatment of
cancer by radiation.

## Key facts

- **NIH application ID:** 3179934
- **Project number:** 2R01CA040245-04A1
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **SAM.gov UEI:** LL8GLEVH6MG3
- **Principal Investigator:** ROY ROWLEY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $150,013
- **Award type:** 2
- **Project period:** 1985-01-15 → 1991-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3179934

## Citation

> US National Institutes of Health, RePORTER application 3179934, RADIATION-INDUCED G2-ARREST AND PROTEIN SYNTHESIS (2R01CA040245-04A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3179934. Licensed CC0.

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