An award is made to Clemson University to enable a five to ten-fold increase in the number of different things that can be measured in a mixture simultaneously (multiplexing), using widely available, affordable, and reliable methods. This project will have broader impacts related to enhanced scientific access to highly-multiplexed experimental capabilities. It will provide training for the next generation of scientists by engaging undergraduates and high school students from a variety of disciplinary backgrounds and by mentoring doctoral students to carry out the work. Findings will be integrated with courses at Clemson University and with continued implementation of new student learning assessment methods. They will be available by open-source access of results, protocols, raw data, and analysis tools, and by public outreach through the Clemson University student chapters of scientific societies. Fluorescent reagents are a workhorse across the biological sciences. Conventional fluorescence multiplexing is limited to about four colors simultaneously, with recent advances reporting from 20 to 40 colors. This project will enable about 200 colors, which is a five-ten-fold increase. The approach is compatible with other current methods, which could expand multiplexing another five-ten-fold, to between 1,000 and 2,000 measurements. Increased measurement multiplexing usually enables breakthrough advances in biological understanding. The recent availability of compatible and aff