The broader/commercial impact of this Small Business Innovation Research (SBIR) Phase II project will be the establishment of a cost-effective, domestic biomanufacturing platform for producing key chemical ingredients from alternate sources. This innovation addresses the national need for more resilient supply chains by reducing reliance on foreign chemical production. The technology is designed to repurpose existing bioethanol production infrastructure, providing a pathway to revitalize these assets and support economic development and job creation in rural agricultural communities. By creating a sustainable, bio-based alternative for chemicals used in countless consumer products, from personal care items to paints and coatings, this project will deliver significant economic and societal benefits. The focus of this project is a chemical intermediate that serves as a feedstock used to manufacture a wide range of acrylic-based materials. Furthermore, the project will advance scientific and technological understanding by demonstrating a new, more energy-efficient method for engineering microorganisms to produce valuable chemicals without the need for oxygen, a long-standing challenge in the field of industrial biotechnology. The proposed project addresses the key technical and economic barriers that have historically limited the anaerobic, or oxygen-free, bio-production of valuable organic acids. The primary challenge is the large amount of cellular energy required to produc